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Abstract

We present a strategy to design and construct self-assembling DNA nanostructures that define intricate curved surfaces in three-dimensional (3D) space using the DNA origami folding technique. Double-helical DNA is bent to follow the rounded contours of the target object, and potential strand crossovers are subsequently identified. Concentric rings of DNA are used to generate in-plane curvature, constrained to 2D by rationally designed geometries and crossover networks. Out-of-plane curvature is introduced by adjusting the particular position and pattern of crossovers between adjacent DNA double helices, whose conformation often deviates from the natural, B-form twist density. A series of DNA nanostructures with high curvature—such as 2D arrangements of concentric rings and 3D spherical shells, ellipsoidal shells, and a nanoflask—were assembled.
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Published In

Science
Volume 332Issue 602715 April 2011
Pages: 342 - 346

History

Received: 18 January 2011
Accepted: 4 March 2011

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The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA.
Suchetan Pal
The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA.
Jeanette Nangreave
The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA.
Zhengtao Deng
The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA.
The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA.
The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA.

Notes

*To whom correspondence should be addressed. E-mail: [email protected] (H.Y.); [email protected] (D.H.); [email protected] (Y.L.)

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Science
Volume 332|Issue 6027
15 April 2011
Submission history
Received:18 January 2011
Accepted:4 March 2011
Published in print:15 April 2011
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