TIR domains of plant immune receptors are NAD + -cleaving enzymes that promote cell death

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Volume 365 | Issue 6455
23 August 2019

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Received: 28 February 2019

Accepted: 10 July 2019

Published in print: 23 August 2019

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Acknowledgments

We thank S. Grant and M. Redinbo for careful reading and discussion of the manuscript. We thank B. Staskawicz for sharing N. benthamiana eds1 and nrg1 mutants. We thank B. Kobe for sharing the sequence of the SARM1 SAM domain pentamutant. We thank K. Simburger and T. Fahrner for technical assistance. We thank C. Argueso and D. Bush for use of facilities. We thank B. Staskawicz, K. H. Sohn, and F. El Kasmi for plasmids. Funding: This work was supported by the National Science Foundation (grant IOS-1758400 to J.L.D. and M.T.N.) and National Institutes of Health (grants GM107444 to J.L.D., RF1AG013730 to J.M., and R01NS087632 to J.M. and A.D.). J.L.D. is a Howard Hughes Medical Institute (HHMI) Investigator. M.T.N. was supported by startup funds from Colorado State University. R.G.A. was supported by an NIH Ruth L. Kirschstein NRSA fellowship (F32GM108226). K.E. was an HHMI Medical Research Fellow. F.M. is supported by a grant from the Gordon and Betty Moore Foundation (GBMF4725) to the Two Blades Foundation. Author contributions: L.W., K.E., R.G.A., J.M., J.L.D., and M.T.N. conceived and designed the study. L.W., K.E., R.G.A., Y.S., F.M., E.C., and M.T.N. generated data. L.W., K.E., J.M., J.L.D., and M.T.N. wrote the initial manuscript. All authors edited the final version of the manuscript. Competing interests: J.M. and Y.S. may derive income from licensing technology to ChromaDex. J.M. and A.D. are cofounders and scientific advisors of Disarm Therapeutics. K.E., Y.S., A.D., and J.M. may derive income from licensing of technology to Disarm Therapeutics. Data and materials availability: All data are available in the manuscript or the supplementary materials.

Authors

Affiliations

Li Wan^* https://orcid.org/0000-0002-0839-3748

Department of Biology and Howard Hughes Medical Institute, University of North Carolina, Chapel Hill, NC 27599, USA.

Kow Essuman^* https://orcid.org/0000-0002-6781-7248

Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110, USA.

Ryan G. Anderson

Department of Biology and Howard Hughes Medical Institute, University of North Carolina, Chapel Hill, NC 27599, USA.

Yo Sasaki https://orcid.org/0000-0003-0024-0031

Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110, USA.

Freddy Monteiro https://orcid.org/0000-0002-9080-6715

Department of Biology and Howard Hughes Medical Institute, University of North Carolina, Chapel Hill, NC 27599, USA.

Center for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, 08193 Barcelona, Spain.

Eui-Hwan Chung https://orcid.org/0000-0002-5048-8142

Department of Biology and Howard Hughes Medical Institute, University of North Carolina, Chapel Hill, NC 27599, USA.

Erin Osborne Nishimura https://orcid.org/0000-0002-4313-4573

Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA.

Aaron DiAntonio https://orcid.org/0000-0002-7262-0968

Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Needleman Center for Neurometabolism and Axonal Therapeutics, Washington University School of Medicine, St. Louis, MO 63110, USA.

Jeffrey Milbrandt^† https://orcid.org/0000-0002-5477-7689 [email protected]

Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110, USA.

Needleman Center for Neurometabolism and Axonal Therapeutics, Washington University School of Medicine, St. Louis, MO 63110, USA.

McDonnell Genome Institute, Washington University School of Medicine, St. Louis, MO 63108, USA.

Jeffery L. Dangl^† https://orcid.org/0000-0003-3199-8654 [email protected]

Department of Biology and Howard Hughes Medical Institute, University of North Carolina, Chapel Hill, NC 27599, USA.

Marc T. Nishimura^† https://orcid.org/0000-0003-4666-6900 [email protected]

Department of Biology, Colorado State University, Fort Collins, CO 80523, USA.