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Self-organization of stem cells into embryos: A window on early mammalian development

Marta N. Shahbazi https://orcid.org/0000-0002-1599-5747 [email protected], Eric D. Siggia https://orcid.org/0000-0001-7482-1854 [email protected], and Magdalena Zernicka-Goetz https://orcid.org/0000-0002-7004-2471 [email protected]
Science • 7 Jun 2019 • Vol 364, Issue 6444 • pp. 948-951 • DOI: 10.1126/science.aax0164
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Abstract

Embryonic development is orchestrated by robust and complex regulatory mechanisms acting at different scales of organization. In vivo studies are particularly challenging for mammals after implantation, owing to the small size and inaccessibility of the embryo. The generation of stem cell models of the embryo represents a powerful system with which to dissect this complexity. Control of geometry, modulation of the physical environment, and priming with chemical signals reveal the intrinsic capacity of embryonic stem cells to make patterns. Adding the stem cells for the extraembryonic lineages generates three-dimensional models that are more autonomous from the environment and recapitulate many features of the pre- and postimplantation mouse embryo, including gastrulation. Here, we review the principles of self-organization and how they set cells in motion to create an embryo.
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Volume 364 | Issue 6444
7 June 2019

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Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
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Acknowledgments

We are grateful to N. Rivron, B. Sozen, D. Turner, A. Martinez-Arias, J. Fu, M. Simunovic, A. Yoney, R. Nusse, D. ten Berge, and W. Koole for providing images for Fig. 2. Funding: M.N.S received funding from an Early Career Leverhulme Trust fellowship and an Advanced EMBO fellowship. Work in the laboratory of M.Z-G. is funded by the Wellcome Trust (207415/Z/17/Z) and the European Research Council (ERC grant 669198). Work of E.D.S. is funded by NIH grant GM101653. Author contributions: M.N.S., E.D.S., and M.Z.-G. conceptualized, wrote, and edited the manuscript. Competing interests: E.D.S. is a cofounder of Rumi Scientific.

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Marta N. Shahbazi*,† https://orcid.org/0000-0002-1599-5747 [email protected]
Mammalian Embryo and Stem Cell Group, Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK.
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Eric D. Siggia*,† https://orcid.org/0000-0001-7482-1854 [email protected]
Center for Studies in Physics and Biology, The Rockefeller University, New York, NY 10065, USA.
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Magdalena Zernicka-Goetz*,† https://orcid.org/0000-0002-7004-2471 [email protected]
Mammalian Embryo and Stem Cell Group, Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK.
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Funding Information

Wellcome Trust: 207415/Z/17/Z
NIH National Institute of Health: GM101653

Notes

*
These authors contributed equally to this work.
†Corresponding author. Email: [email protected] (M.N.S.); [email protected] (E.D.S.); [email protected] (M.Z.-G.)

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