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Abstract

An approach for genome analysis based on sequencing and assembly of unselected pieces of DNA from the whole chromosome has been applied to obtain the complete nucleotide sequence (1,830,137 base pairs) of the genome from the bacterium Haemophilus influenzae Rd. This approach eliminates the need for initial mapping efforts and is therefore applicable to the vast array of microbial species for which genome maps are unavailable. The H. influenzae Rd genome sequence (Genome Sequence DataBase accession number L42023) represents the only complete genome sequence from a free-living organism.

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References

Adams, M. D., Automated DNA Sequencing and Analysis (1994).
Adams, M. D., Nature 368: 474 (1994).
ADAMS, M.D., NATURE 355: 632 (1992).
ADAMS, M.D., NATURE 368: 474 (1994).
ADAMS, M.D., SCIENCE 252: 1651 (1991).
ALBRIGHT, L.M., ANNUAL REVIEW OF GENETICS 23: 311 (1989).
ALTSCHUL, S.F., JOURNAL OF MOLECULAR BIOLOGY 215: 403 (1990).
BANKIER, A.T., DNA SEQUENCE 2: 1 (1991).
BAROUKI, R, REEXAMINATION OF PHENOTYPIC DEFECTS IN REC-1 AND REC-2 MUTANTS OF HEMOPHILUS-INFLUENZAE RD, JOURNAL OF BACTERIOLOGY 163: 629 (1985).
BODMER, W.F., REV INVEST CLIN S: 3 (1994).
BORODOVSKY, M, GENMARK - PARALLEL GENE RECOGNITION FOR BOTH DNA STRANDS, COMPUTERS & CHEMISTRY 17: 123 (1993).
BRUTLAG, D.L., BLAZE (TM) - AN IMPLEMENTATION OF THE SMITH-WATERMAN SEQUENCE COMPARISON ALGORITHM ON A MASSIVELY-PARALLEL COMPUTER, COMPUTERS & CHEMISTRY 17: 203 (1993).
BURLAND, V, DNA-SEQUENCE AND ANALYSIS OF 136 KILOBASES OF THE ESCHERICHIA-COLI GENOME - ORGANIZATIONAL SYMMETRY AROUND THE ORIGIN OF REPLICATION, GENOMICS 16: 551 (1993).
CHANDLER, M.S., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 89: 1616 (1992).
CLAVERIE, J.M., DETECTING FRAME SHIFTS BY AMINO-ACID-SEQUENCE COMPARISON, JOURNAL OF MOLECULAR BIOLOGY 234: 1140 (1993).
DANNER, D.B., AN 11-BASE-PAIR SEQUENCE DETERMINES THE SPECIFICITY OF DNA UPTAKE IN HEMOPHILUS TRANSFORMATION, GENE 11: 311 (1980).
DANNER, D.B., CONSTRUCTION OF DNA RECOGNITION SITES ACTIVE IN HEMOPHILUS TRANSFORMATION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 79: 2393 (1982).
DOROCICZ, I.R., THE HAEMOPHILUS-INFLUENZAE ADENYLATE-CYCLASE GENE - CLONING, SEQUENCE, AND ESSENTIAL ROLE IN COMPETENCE, JOURNAL OF BACTERIOLOGY 175: 7142 (1993).
DOUGHERTY, B, unpublished data.
GLASER, P, BACILLUS-SUBTILIS GENOME PROJECT - CLONING AND SEQUENCING OF THE 97KB REGION FROM 325-DEGREES TO 333 DEGREES, MOLECULAR MICROBIOLOGY 10: 371 (1993).
GOEBEL, S.J., THE COMPLETE DNA-SEQUENCE OF VACCINIA VIRUS, VIROLOGY 179: 247 (1990).
GREENER, A, STRATEGIES MOL BIOL 3: 5 (1990).
HARTL, D.L., GENOME RES MOL MED V: 115 (1993).
HENIKOFF, S, AMINO-ACID SUBSTITUTION MATRICES FROM PROTEIN BLOCKS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 89: 10915 (1992).
HONG, G.F., J MOL BIOL 162: 72 (1982).
JOHNSTON, M, COMPLETE NUCLEOTIDE-SEQUENCE OF SACCHAROMYCES-CEREVISIAE CHROMOSOME-VIII, SCIENCE 265: 2077 (1994).
KAHN, M.E., J MEMBRANE BIOL 138: 155 (1984).
KERLAVAGE, A, 26TH P HAW INT C SYS: 585 (1993).
Kerlavage, A. R., IEEE Computers in Medicine and Biology, in press.
KIRKNESS, E.F., GENOMICS 10: 985 (1991).
KLEIN, R.D., JOURNAL OF GENERAL MICROBIOLOGY 113: 409 (1979).
KYTE, J, JOURNAL OF MOLECULAR BIOLOGY 157: 105 (1982).
LANDER, E.S., GENOMICS 2: 231 (1988).
LEE, J.J., ORGANIZATION OF THE HEMOPHILUS-INFLUENZAE RD GENOME, JOURNAL OF BACTERIOLOGY 171: 3016 (1989).
LEVY, J, SEQUENCING THE YEAST GENOME - AN INTERNATIONAL ACHIEVEMENT, YEAST 10: 1689 (1994).
LEWIN, B, GENES 5: CH18 (1994).
LEWIN, B, GENES 5: CH19 (1994).
MASSUNG, R.F., POTENTIAL VIRULENCE DETERMINANTS IN TERMINAL REGIONS OF VARIOLA SMALLPOX VIRUS GENOME, NATURE 366: 748 (1993).
ODA, K, GENE ORGANIZATION DEDUCED FROM THE COMPLETE SEQUENCE OF LIVERWORT MARCHANTIA-POLYMORPHA MITOCHONDRIAL-DNA - A PRIMITIVE FORM OF PLANT MITOCHONDRIAL GENOME, JOURNAL OF MOLECULAR BIOLOGY 223: 1 (1992).
OHYAMA, K, CHLOROPLAST GENE ORGANIZATION DEDUCED FROM COMPLETE SEQUENCE OF LIVERWORT MARCHANTIA-POLYMORPHA CHLOROPLAST DNA, NATURE 322: 572 (1986).
PARKINSON, J.S., COMMUNICATION MODULES IN BACTERIAL SIGNALING PROTEINS, ANNUAL REVIEW OF GENETICS 26: 71 (1992).
PEARSON, W.R., IMPROVED TOOLS FOR BIOLOGICAL SEQUENCE COMPARISON, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 2444 (1988).
REDFIELD, R.J., SXY-1, A HAEMOPHILUS-INFLUENZAE MUTATION CAUSING GREATLY ENHANCED SPONTANEOUS COMPETENCE, JOURNAL OF BACTERIOLOGY 173: 5612 (1991).
RILEY, M, FUNCTIONS OF THE GENE-PRODUCTS OF ESCHERICHIA-COLI, MICROBIOLOGICAL REVIEWS 57: 862 (1993).
SANGER, F, NATURE 2461: 687 (1977).
SMITH, H.O., FREQUENCY AND DISTRIBUTION OF DNA UPTAKE SIGNAL SEQUENCES IN THE HAEMOPHILUS-INFLUENZAE RD GENOME, SCIENCE 269: 538 (1995).
SOFIA, H.J., ANALYSIS OF THE ESCHERICHIA-COLI GENOME .5. DNA-SEQUENCE OF THE REGION FROM 76.0-MINUTES TO 81.5-MINUTES, NUCLEIC ACIDS RESEARCH 22: 2576 (1994).
SULSTON, J, THE C-ELEGANS GENOME SEQUENCING PROJECT - A BEGINNING, NATURE 356: 37 (1992).
TOMB, J.F., NUCLEOTIDE-SEQUENCE OF A CLUSTER OF GENES INVOLVED IN THE TRANSFORMATION OF HAEMOPHILUS-INFLUENZAE RD, GENE 104: 1 (1991).
TOMB, J.F., A PERIPLASMIC PROTEIN DISULFIDE OXIDOREDUCTASE IS REQUIRED FOR TRANSFORMATION OF HAEMOPHILUS-INFLUENZAE RD, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 89: 10252 (1992).
TTOMB, J.F., unpublished data.
UTTERBACK, T.R., unpublished data.
VANHAM, S.M., THE FIMBRIAL GENE-CLUSTER OF HAEMOPHILUS-INFLUENZAE TYPE-B, MOLECULAR MICROBIOLOGY 13: 673 (1994).
WATERMAN, M.S., METHODS IN ENZYMOLOGY 164: 765 (1988).
WEISER, J.N., THE MOLECULAR MECHANISM OF PHASE VARIATION OF H-INFLUENZAE LIPOPOLYSACCHARIDE, CELL 59: 657 (1989).
WILCOX, K.W., ISOLATION AND CHARACTERIZATION OF MUTANTS OF HEMOPHILUS-INFLUENZAE DEFICIENT IN AN ADENOSINE 5'-TRIPHOSPHATE-DEPENDENT DEOXYRIBONUCLEASE ACTIVITY, JOURNAL OF BACTERIOLOGY 122: 443 (1975).
YURA, T, SYSTEMATIC SEQUENCING OF THE ESCHERICHIA-COLI GENOME - ANALYSIS OF THE 0 - 2.4 MIN REGION, NUCLEIC ACIDS RESEARCH 20: 3305 (1992).

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Science
Volume 269 | Issue 5223
28 July 1995

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Published in print: 28 July 1995

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Authors

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Robert D. Fleischmann
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Mark D. Adams
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Owen White
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Rebecca A. Clayton
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Ewen F. Kirkness
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Anthony R. Kerlavage
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Carol J. Bult
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Jean-Francois Tomb
Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Brian A. Dougherty
Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Joseph M. Merrick
State University of New York, Department of Microbiology, Buffalo, NY, 14214, USA.
Keith McKenney
National Institute for Standards and Technology, Gaithersburg, MD 20878, USA.
Granger Sutton
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Will FitzHugh,
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Chris Fields
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Jeannine D. Gocayne
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
John Scott
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Robert Shirley
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Li-lng Liu
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Anna Glodek
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Jenny M. Kelley
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Janice F. Weidman
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Cheryl A. Phillips
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Tracy Spriggs
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Eva Hedblom
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Matthew D. Cotton
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Teresa R. Utterback
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Michael C. Hanna
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
David T. Nguyen
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Deborah M. Saudek
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Rhonda C. Brandon
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Leah D. Fine
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Janice L. Fritchman
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Joyce L. Fuhrmann
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
N. S. M. Geoghagen
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Cheryl L. Gnehm
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Lisa A. McDonald
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Keith V. Small
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Claire M. Fraser
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
Hamilton O. Smith
Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
J. Craig Venter
The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.

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