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Abstract

A pool of synthetic oligonucleotides was prepared based on the amino terminal amino acid sequence of tetanus toxin. This probe hybridized to plasmid DNA isolated from three toxigenic strains of Clostridium tetani but not to plasmid DNA from a nontoxigenic strain. These results show that the structural gene for the toxin is on the plasmid. The pCL1 plasmid from one of the toxigenic strains spontaneously deleted 22 kilobase pairs of DNA to form pCL2. Strains harboring this deleted plasmid are nontoxigenic. However, the probe mixture hybridized to pCL2, indicating that the DNA encoding the amino terminus of the toxin had not been deleted. Restriction endonuclease cleavage maps of pCL1 and pCL2 were constructed and indicate the approximate location and orientation of the structural gene for tetanus toxin.

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References

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Science
Volume 224 | Issue 4651
25 May 1984

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Published in print: 25 May 1984

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Charles W. Finn, Jr.
Division of Bacterial Products, Office of Biologics Research and Review, Center for Drugs and Biologics, Food and Drug Administration, Bethesda, Maryland 20205
Richard P. Silver
Division of Bacterial Products, Office of Biologics Research and Review, Center for Drugs and Biologics, Food and Drug Administration, Bethesda, Maryland 20205
William H. Habig
Division of Bacterial Products, Office of Biologics Research and Review, Center for Drugs and Biologics, Food and Drug Administration, Bethesda, Maryland 20205
M. Carolyn Hardegree
Division of Bacterial Products, Office of Biologics Research and Review, Center for Drugs and Biologics, Food and Drug Administration, Bethesda, Maryland 20205
Gerald Zon
Division of Biochemistry and Biophysics, Office of Biologics Research and Review, Center for Drugs and Biologics, Food and Drug Administration, Bethesda, Maryland 20205
Claude F. Garon
Electron Microscopy Section, Rocky Mountain Laboratory, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840

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