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African Trypanosomes: Cultivation of Animal-Infective Trypanosoma brucei in Vitro

H. Hirumi, J. J. Doyle, and K. HirumiAuthors Info & Affiliations
Science
27 May 1977
Vol 196, Issue 4293
pp. 992-994
DOI: 10.1126/science.558652
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Abstract

Trypanosoma brucei grew in the presence of bovine fibroblast-like cells in Hepes-buffered RPMI 1640 medium with 20 percent fetal bovine serum for more than 220 days at 37°C. The organisms grown in this system were infective to mammalian hosts, retained the morphological and biochemical characteristics of long slender bloodstream forms, and displayed variant-antigen on their surfaces.

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Science
Volume 196 | Issue 4293
27 May 1977

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© 1977.

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Published in print: 27 May 1977

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H. Hirumi
International Laboratory for Research on Animal Diseases, Nairobi, Kenya
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J. J. Doyle
International Laboratory for Research on Animal Diseases, Nairobi, Kenya
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K. Hirumi
International Laboratory for Research on Animal Diseases, Nairobi, Kenya
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  • H. Hirumi
  • et al.
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African Trypanosomes: Cultivation of Animal-Infective Trypanosoma brucei in Vitro.Science196,992-994(1977).DOI:10.1126/science.558652

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