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The Fos Protein Complex Is Associated with DNA in Isolated Nuclei and Binds to DNA Cellulose

Lidia C. Sambucetti and Tom Curran
Science • 12 Dec 1986 • Vol 234, Issue 4782 • pp. 1417-1419 • DOI: 10.1126/science.3491427
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Abstract

The properties of the viral and cellular fos proteins (Fos) were investigated as a first step toward understanding the function of the fos gene. Treatment of nuclei with salt and nonionic detergents solubilized a complex that contained Fos together with several other cellular proteins. The majority of the Fos protein complex was released from isolated nuclei incubated in the presence of deoxyribonuclease I or micrococcal nuclease but not with ribonuclease A, suggesting that Fos is associated with chromatin. This hypothesis is supported by the finding that Fos protein from native or denatured nuclear extracts exhibited DNA-binding activity in vitro. These results suggest that Fos is involved in the regulation of gene expression.

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Published In

Science
Volume 234 | Issue 4782
12 December 1986

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© 1986.

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Published in print: 12 December 1986

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Lidia C. Sambucetti
Department of Molecular Oncology, Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
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Tom Curran
Department of Molecular Oncology, Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
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Cited by
    • Benjamin Rusak,
    • Harold A. Robertson,
    • William Wisden,
    • Stephen P. Hunt,
    Light Pulses that Shift Rhythms Induce Gene Expressions in the Suprachiasmatic Nucleus, Science, 248, 4960, (1237-1240), (1990)./doi/10.1126/science.2112267
    Abstract
    • S. M. Sagar,
    • F. R. Sharp,
    • T. Curran,
    Expression of c-fos Protein in Brain: Metabolic Mapping at the Cellular Level, Science, 240, 4857, (1328-1331), (1988)./doi/10.1126/science.3131879
    Abstract
    • Frank J. Rauscher,
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