Start Sites of Bidirectional DNA Synthesis at the Human Lamin B2 Origin
Abstract
The initiation sites of bidirectional synthesis at the DNA replication origin located at the 3′ end of the human lamin B2 gene were investigated. RNA-primed nascent DNA molecules were subjected to second-strand synthesis with appropriate primers, amplified by ligation-mediated polymerase chain reaction, and size fractionated. Evidence for precise start sites was obtained. Exploration of close to 1 kilobase, coupled to inhibition of Okazaki fragment synthesis, demonstrates that the leading strands initiate at precise nucleotides on either helix, overlapping by three base pairs, within the area bound to a protein complex possibly analogous to the prereplicative complex of yeast.
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Published In
Science
Volume 287 | Issue 5460
17 March 2000
17 March 2000
Submission history
Received: 6 October 1999
Accepted: 2 February 2000
Published in print: 17 March 2000
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