Histone H5 in the Control of DNA Synthesis and Cell Proliferation
Abstract
The linker histones (H1, H5, H1°) are involved in the condensation of chromatin into the 30-nanometer fiber. This supranucleosome organization correlates with the resting state of chromatin, and it is therefore possible that the linker histones play an active role in the control of chromatin activity. The effect of H5 has been directly determined by expression of an inducible transfected H5 gene in rat sarcoma cells, which do not produce H5. Transfection resulted in the reversible inhibition of DNA replication and arrest of cells in G1, at which time H5 concentrations approached that of terminally differentiated avian erythrocytes. The arrest of proliferation was accompanied by specific changes in gene expression probably related to the cell cycle block. The selectivity of these effects suggest that H5 plays an active role in the control of DNA replication and cell proliferation.
References
AFFOLTER, M, TRANSCRIPTION UNIT OF THE CHICKEN HISTONE H-5 GENE AND MAPPING OF H-5 PRE-MESSENGER-RNA SEQUENCES, JOURNAL OF BIOLOGICAL CHEMISTRY 261: 11496 (1986).
AFFOLTER, M, REGULATION OF HISTONE AND BETA-A-GLOBIN GENE-EXPRESSION DURING DIFFERENTIATION OF CHICKEN ERYTHROID-CELLS, MOLECULAR AND CELLULAR BIOLOGY 7: 3663 (1987).
ALLAN, J, THE STRUCTURE OF HISTONE-H1 AND ITS LOCATION IN CHROMATIN, NATURE 288: 675 (1980).
ALONSO, A, EARLY INCREASE IN HISTONE-H1-DEGREES MESSENGER-RNA DURING DIFFERENTIATION OF F9 CELLS TO PARIETAL ENDODERM, EMBO JOURNAL 7: 3003 (1988).
APPELS, R, SYNTHESIS AND TURNOVER OF DNA-BOUND HISTONE DURING MATURATION OF AVIAN RED BLOOD-CELLS, JOURNAL OF MOLECULAR BIOLOGY 70: 425 (1972).
BILLET, M.A., STUDY OF QUANTITATIVE VARIATION OF HISTONES, AND THEIR RELATIONSHIP TO RNA SYNTHESIS, DURING ERYTHROPOIESIS IN ADULT CHICKEN, EUROPEAN JOURNAL OF BIOCHEMISTRY 28: 451 (1972).
CHABANAS, A, EFFECTS OF BUTYRIC-ACID ON CELL-CYCLE REGULATION AND INDUCTION OF HISTONE H1-DEGREES IN MOUSE CELLS AND TISSUE-CULTURE INDUCIBILITY OF H1-DEGREES IN THE LATE S-G2 PHASE OF THE CELL-CYCLE, JOURNAL OF MOLECULAR BIOLOGY 183: 141 (1985).
CIDLOWSKI, J.A., GLUCOCORTICOID RECEPTORS AND THE CELL-CYCLE - EVIDENCE THAT THE ACCUMULATION OF GLUCOCORTICOID RECEPTORS DURING THE S-PHASE OF THE CELL-CYCLE IS DEPENDENT ON RIBONUCLEIC-ACID AND PROTEIN-SYNTHESIS, ENDOCRINOLOGY 110: 1653 (1982).
CIDLOWSKI, J.A., ALTERATION IN GLUCOCORTICOID BINDING-SITE NUMBER DURING CELL-CYCLE IN HELA-CELLS, NATURE 266: 643 (1977).
COLE, M.D., THE MYC ONCOGENE - ITS ROLE IN TRANSFORMATION AND DIFFERENTIATION, ANNUAL REVIEW OF GENETICS 20: 361 (1986).
CRABTREE, G.R., GLUCOCORTICOIDS AND LYMPHOCYTES .2. CELL CYCLE-DEPENDENT CHANGES IN GLUCOCORTICOID RECEPTOR CONTENT, JOURNAL OF IMMUNOLOGY 125: 13 (1980).
DALTON, S, TRANSCRIPTION OF THE HISTONE-H5 GENE IS NOT S-PHASE REGULATED, MOLECULAR AND CELLULAR BIOLOGY 6: 601 (1986).
DANNA, J.A., SYNTHESES AND MODULATIONS IN THE CHROMATIN CONTENTS OF HISTONE-H10 AND HISTONE-H1 DURING G1-PHASE AND S-PHASE IN CHINESE-HAMSTER CELLS, BIOCHEMISTRY 21: 3991 (1982).
ENGEL, J.D., A CHICKEN HISTONE H-3 GENE CONTAINS INTERVENING SEQUENCES, NATURE 297: 434 (1982).
FERRARI, S, CODING SEQUENCE AND GROWTH-REGULATION OF THE HUMAN VIMENTIN GENE, MOLECULAR AND CELLULAR BIOLOGY 6: 3614 (1986).
GJERSET, R, DEVELOPMENTAL AND HORMONAL-REGULATION OF PROTEIN H-1-DEGREE IN RODENTS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 79: 2333 (1982).
GORMAN, C, HIGH-EFFICIENCY DNA-MEDIATED TRANSFORMATION OF PRIMATE CELLS, SCIENCE 221: 551 (1983).
GRAHAM, F.L., NEW TECHNIQUE FOR ASSAY OF INFECTIVITY OF HUMAN ADENOVIRUS 5 DNA, VIROLOGY 52: 456 (1973).
HEREFORD, L, PERIODIC TRANSCRIPTION OF YEAST HISTONE GENES, CELL 30: 305 (1982).
HIRSCHHORN, R.R., CELL-CYCLE-SPECIFIC CDNAS FROM MAMMALIAN-CELLS TEMPERATURE SENSITIVE FOR GROWTH, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 81: 6004 (1984).
KUMAR, N.M., THE BINDING OF HISTONE-H1 AND HISTONE-H5 TO CHROMATIN IN CHICKEN ERYTHROCYTE NUCLEI, NUCLEIC ACIDS RESEARCH 8: 3535 (1980).
LAEMMLI, U.K., CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4, NATURE 227: 680 (1970).
LAND, H, TUMORIGENIC CONVERSION OF PRIMARY EMBRYO FIBROBLASTS REQUIRES AT LEAST 2 COOPERATING ONCOGENES, NATURE 304: 596 (1983).
LANGER, S.J., NEGATIVE REGULATION OF TRANSCRIPTION INVITRO BY A GLUCOCORTICOID RESPONSE ELEMENT IS MEDIATED BY A TRANS-ACTING FACTOR, MOLECULAR AND CELLULAR BIOLOGY 8: 3872 (1988).
Lennox, R. W., Histone Genes, Organization and Regulation: 373 (1984).
MIESFELD, R, GENETIC COMPLEMENTATION OF A GLUCOCORTICOID RECEPTOR DEFICIENCY BY EXPRESSION OF CLONED RECEPTOR CDNA, CELL 46: 389 (1986).
NUDEL, U, THE NUCLEOTIDE-SEQUENCE OF THE RAT CYTOPLASMIC BETA-ACTIN GENE, NUCLEIC ACIDS RESEARCH 11: 1759 (1983).
Pehrson, J., Nature 285:43 (1980).
PEHRSON, J.R., BIOCHEMISTRY-US 21: 456 (1982).
PUIGDOMENECH, P, ISOLATION OF A 167 BASEPAIR CHROMATOSOME CONTAINING A PARTIALLY DIGESTED HISTONE-H5, FEBS LETTERS 154: 151 (1983).
ROZALSKI, M, MONOCLONAL-ANTIBODIES AGAINST HISTONE-H5 - EPITOPE MAPPING AND BINDING TO CHROMATIN, JOURNAL OF BIOLOGICAL CHEMISTRY 260: 14379 (1985).
RUIZCARRILLO, A, STABILITY AND REVERSIBILITY OF HIGHER ORDERED STRUCTURE OF INTERPHASE CHROMATIN - CONTINUITY OF DEOXYRIBONUCLEIC-ACID IS NOT REQUIRED FOR MAINTENANCE OF FOLDED STRUCTURE, BIOCHEMISTRY 19: 2544 (1980).
RUIZCARRILLO, A, CHANGES IN HISTONE ACETYL CONTENT AND IN NUCLEAR NON-HISTONE PROTEIN COMPOSITION OF AVIAN ERYTHROID-CELLS AT DIFFERENT STAGES OF MATURATION, JOURNAL OF BIOLOGICAL CHEMISTRY 249: 7358 (1974).
RUIZCARRILLO, A, GENOMIC ORGANIZATION OF THE GENES-CODING FOR THE 6 MAIN HISTONES OF THE CHICKEN - COMPLETE SEQUENCE OF THE H5-GENE, JOURNAL OF MOLECULAR BIOLOGY 170: 843 (1983).
RUIZCARRILLO, A, unpublished data.
STAYNOV, D.Z., FOOTPRINTING OF LINKER HISTONES-H5 AND HISTONES-H1 ON THE NUCLEOSOME, EMBO JOURNAL 7: 3685 (1988).
SUGARMAN, B.J., GENOMIC ORGANIZATION, DNA-SEQUENCE, AND EXPRESSION OF CHICKEN EMBRYONIC HISTONE GENES, JOURNAL OF BIOLOGICAL CHEMISTRY 258: 9005 (1983).
SVOBODA, J, PRESENCE OF CHICKEN TUMOUR VIRUS IN THE SARCOMA OF THE ADULT RAT INOCULATED AFTER BIRTH WITH ROUS SARCOMA TISSUE, NATURE 186: 980 (1960).
THOMAS, J.O., EXCHANGE OF HISTONE-H1 AND HISTONE-H5 BETWEEN CHROMATIN FRAGMENTS - A PREFERENCE OF H-5 FOR HIGHER-ORDER STRUCTURES, EUROPEAN JOURNAL OF BIOCHEMISTRY 134: 109 (1983).
TORRESMARTINEZ, S, NUCLEOSOMES CONTAINING HISTONES H-1 OR H-5 ARE CLOSELY INTERSPERSED IN CHROMATIN, NUCLEIC ACIDS RESEARCH 10: 2323 (1982).
URBAN, M.K., CHANGES IN NUCLEOSOMAL CORE HISTONE VARIANTS DURING CHICKEN DEVELOPMENT AND MATURATION, DEVELOPMENTAL BIOLOGY 95: 421 (1983).
WEINTRAUB, H, HISTONE-H1-DEPENDENT CHROMATIN SUPERSTRUCTURES AND THE SUPPRESSION OF GENE ACTIVITY, CELL 38: 17 (1984).
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Volume 245 | Issue 4913
7 July 1989
7 July 1989
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