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Abstract

The mouse albumin gene promoter has six closely spaced binding sites for nuclear proteins that are located between the TATA motif and nucleotide position -170. In vitro transcription with liver or spleen nuclear extracts of templates containing either mutated or polymerized albumin promoter elements establishes a hierarchy of the different protein binding sites for tissue-specific albumin gene transcription. The HNF-1 and C/EBP binding sites strongly activate transcription in a tissue-specific manner. The NF-Y binding site has a lower activation potential and is less specific, being equally efficient in liver and spleen nuclear extracts. The remaining elements are relatively weak activator sites.

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Published In

Science
Volume 244 | Issue 4902
21 April 1989

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Published in print: 21 April 1989

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Pascal Maire
Département de Biologie Moléculaire, Sciences II, Geneva, Switzerland.
Jérôme Wuarin
Département de Biologie Moléculaire, Sciences II, Geneva, Switzerland.
Ueli Schibler
Département de Biologie Moléculaire, Sciences II, Geneva, Switzerland.

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