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Functional Evidence for Indirect Recognition of G·U in tRNAAla by Alanyl-tRNA Synthetase

K. Gabriel, Jay Schneider, and William H. McClain
Science • 12 Jan 1996 • Vol 271, Issue 5246 • pp. 195-197 • DOI: 10.1126/science.271.5246.195
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Abstract

The structural features of the G⋅U wobble pair in Escherichia coli alanine transfer RNA (tRNAAla) that are associated with aminoacylation by alanyl-tRNA synthetase (AlaRS) were investigated in vivo for wild-type tRNAAla and mutant tRNAs with G⋅U substitutions. tRNAAla with G⋅U, C⋅A, or G⋅A gave similar amounts of charged tRNAAla and supported viability of E. coli lacking chromosomal tRNAAla genes. tRNAAla with G⋅C was inactive. Recognition of G⋅U by AlaRS thus requires more than the functional groups on G⋅U in a regular helix and may involve detection of a helical distortion.

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Published In

Science
Volume 271 | Issue 5246
12 January 1996

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© 1996 American Association for the Advancement of Science.

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Published in print: 12 January 1996

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K. Gabriel
Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA.
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Jay Schneider
Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA.
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William H. McClain(1)
Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA.
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Notes

(1) To whom correspondence should be addressed.

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Cited by
    • Margaret E. Saks,
    • Jeffrey R. Sampson,
    • John Abelson,
    Evolution of a Transfer RNA Gene Through a Point Mutation in the Anticodon, Science, 279, 5357, (1665-1670), (2021)./doi/10.1126/science.279.5357.1665
    Abstract

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