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Abstract

T lymphocyte chemotactic factor (TCF) was purified to homogeneity from the conditioned media of phytohemagglutinin-stimulated human blood mononuclear leukocytes by a sequence of chromatography procedures. The amino-terminal amino acid sequence of the purified TCF showed identity with neutrophil-activating protein (NAP-1). Both TCF and recombinant NAP-1 (rNAP-1) were chemotactic for neutrophils and T lymphocytes in vitro supporting the identity of TCF with NAP-1. Injection of rNAP-1 into lymphatic drainage areas of lymph nodes in Fisher rats caused accelerated emigration of only lymphocytes in high endothelial venules. Intradermal injection of rNAP-1 caused dose-dependent accumulation of neutrophils and lymphocytes.

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References

FALK, W, A 48-WELL MICRO CHEMOTAXIS ASSEMBLY FOR RAPID AND ACCURATE MEASUREMENT OF LEUKOCYTE MIGRATION, JOURNAL OF IMMUNOLOGICAL METHODS 33: 239 (1980).
GAWKRODGER, D.J., PHENOTYPIC CHARACTERIZATION OF THE EARLY CELLULAR-RESPONSES IN ALLERGIC AND IRRITANT CONTACT-DERMATITIS, CLINICAL AND EXPERIMENTAL IMMUNOLOGY 66: 590 (1986).
HASKARD, D, PHORBOL ESTER-STIMULATED LYMPHOCYTES-T SHOW ENHANCED ADHESION TO HUMAN-ENDOTHELIAL CELL MONOLAYERS, JOURNAL OF IMMUNOLOGY 137: 1429 (1986).
JULIUS, M.H., RAPID METHOD FOR ISOLATION OF FUNCTIONAL THYMUS-DERIVED MURINE LYMPHOCYTES, EUROPEAN JOURNAL OF IMMUNOLOGY 3: 645 (1973).
KUPPER, T.S., HUMAN KERATINOCYTES CONTAIN MESSENGER-RNA INDISTINGUISHABLE FROM MONOCYTE INTERLEUKIN-1-ALPHA AND BETA-MESSENGER RNA - KERATINOCYTE EPIDERMAL-CELL DERIVED THYMOCYRE-ACTIVATING FACTOR IS IDENTICAL TO INTERLEUKIN-1, JOURNAL OF EXPERIMENTAL MEDICINE 164: 2095 (1986).
LARSEN, C.G., EPIDERMIS AND LYMPHOCYTE INTERACTIONS DURING A TUBERCULIN SKIN REACTION .1. INCREASED ETAF IL-1 LIKE ACTIVITY, EXPRESSION OF TISSUE ANTIGENS AND MIXED SKIN LYMPHOCYTE-REACTIVITY, ARCHIVES OF DERMATOLOGICAL RESEARCH 280: 83 (1988).
LARSEN, C.G., EPIDERMIS AND LYMPHOCYTE INTERACTIONS DURING AN ALLERGIC PATCH TEST REACTION INCREASED ACTIVITY OF ETAF/IL-1, EPIDERMAL DERIVED LYMPHOCYTE CHEMOTACTIC FACTOR AND MIXED SKIN LYMPHOCYTE-REACTIVITY IN PERSONS WITH TYPE-IV ALLERGY, JOURNAL OF INVESTIGATIVE DERMATOLOGY 90: 230 (1988).
LARSEN, C.G., unpublished data.
MATSUSHIMA, K, MOLECULAR-CLONING OF A HUMAN MONOCYTE-DERIVED NEUTROPHIL CHEMOTACTIC FACTOR (MDNCF) AND THE INDUCTION OF MDNCF MESSENGER-RNA BY INTERLEUKIN-1 AND TUMOR NECROSIS FACTOR, JOURNAL OF EXPERIMENTAL MEDICINE 167: 1883 (1988).
PARROTT, DMV, PROG ALLERGY 28: 193 (1981).
RANNIE, G.H., RECIRCULATION OF LYMPHOCYTES - ITS ROLE IN IMPLEMENTING IMMUNE-RESPONSES IN SKIN, LYMPHOLOGY 11: 193 (1978).
SAMANTA, A, unpublished data.
SAVAGNER, P, HOMING OF HEMATOPOIETIC PRECURSOR CELLS TO THE EMBRYONIC THYMUS - CHARACTERIZATION OF AN INVASIVE MECHANISM INDUCED BY CHEMOTACTIC PEPTIDES, JOURNAL OF CELL BIOLOGY 103: 2715 (1986).
VANDAMME, J, A NOVEL, NH2-TERMINAL SEQUENCE CHARACTERIZED HUMAN MONOKINE POSSESSING NEUTROPHIL CHEMOTACTIC, SKIN-REACTIVE, AND GRANULOCYTOSIS-PROMOTING ACTIVITY, JOURNAL OF EXPERIMENTAL MEDICINE 167: 1364 (1988).
YOSHIMURA, T, NEUTROPHIL CHEMOTACTIC FACTOR PRODUCED BY LIPOPOLYSACCHARIDE (LPS)-STIMULATED HUMAN-BLOOD MONONUCLEAR LEUKOCYTES - PARTIAL CHARACTERIZATION AND SEPARATION FROM INTERLEUKIN-1 (IL-1), JOURNAL OF IMMUNOLOGY 139: 788 (1987).
YOSHIMURA, T, PURIFICATION OF A HUMAN MONOCYTE-DERIVED NEUTROPHIL CHEMOTACTIC FACTOR THAT HAS PEPTIDE SEQUENCE SIMILARITY TO OTHER HOST DEFENSE CYTOKINES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 84: 9233 (1987).
ZACHARIAE, C, EPIDERMAL LYMPHOCYTE CHEMOTACTIC FACTOR SPECIFICALLY ATTRACTS OKT4-POSITIVE LYMPHOCYTES, ARCHIVES OF DERMATOLOGICAL RESEARCH 280: 354 (1988).
ZIGMOND, S.H., LEUKOCYTE LOCOMOTION AND CHEMOTAXIS - NEW METHODS FOR EVALUATION AND DEMONSTRATION OF A CELL-DERIVED CHEMOTACTIC FACTOR, JOURNAL OF EXPERIMENTAL MEDICINE 137: 387 (1973).

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Science
Volume 243 | Issue 4897
17 March 1989

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Published in print: 17 March 1989

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Christian Grønhøj Larsen
Laboratory of Molecular Imnmunorepulation, Biological Response Modifiers Program, Division of Cancer Treatment, National Cancer Institute, Frederick, MD 21701.
Arthur O. Anderson
Laboratory of Respiratory and Mucosal Immunity, Disease Assessment Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21701.
Ettore Appella
Laboratory of Cell Biology, National Cancer Institute, Bethesda, MD 20892.
Joost J. Oppenheim
Laboratory of Molecular Imnmunorepulation, Biological Response Modifiers Program, Division of Cancer Treatment, National Cancer Institute, Frederick, MD 21701.
Kouji Matsushima
Laboratory of Molecular Imnmunorepulation, Biological Response Modifiers Program, Division of Cancer Treatment, National Cancer Institute, Frederick, MD 21701.

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