Inhibition of Antigen-Induced Lymphocyte Proliferation by Tat Protein from HIV-1
Abstract
The purified human immunodeficiency virus type-1 (HIV-1) Tat protein inhibited lymphocyte proliferation induced by tetanus toxoid or Candida antigens by 66 to 97% at nanomolar concentrations of Tat. In contrast, Tat did not cause a significant reduction of lymphocyte proliferation in response to mitogens such as phytohemagglutinin or pokeweed mitogen. Inhibition was blocked by oxidation of the cysteine-rich region of Tat or by incubation with an antibody to Tat before the assay. A synthetic Tat peptide (residues 1 to 58) also inhibited antigen-stimulated proliferation. Experiments with H9 and U937 cell lines showed that Tat can easily enter both lymphocytes and monocytes. The specific inhibition of antigen-induced lymphocyte proliferation by Tat mimics the effect seen with lymphocytes from HIV-infected individuals and suggests that Tat might directly contribute to the immunosuppression associated with HIV infection.
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Published In
Science
Volume 246 | Issue 4937
22 December 1989
22 December 1989
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© 1989.
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Published in print: 22 December 1989
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Cited by
- Induction of Apoptosis in Uninfected Lymphocytes by HIV-1 Tat Protein, Science, 268, 5209, (429-431), (1995)./doi/10.1126/science.7716549
- Participation of Tyrosine Phosphorylation in the Cytopathic Effect of Human Immunodeficiency Virus-1, Science, 256, 5056, (542-545), (1992)./doi/10.1126/science.1570514
- Inhibition of HIV Replication in Acute and Chronic Infections in Vitro by a Tat Antagonist, Science, 254, 5039, (1799-1802), (1991)./doi/10.1126/science.1763331
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