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Research Article

Biophysical and Molecular Mechanisms of Shaker Potassium Channel Inactivation

Science
26 Oct 1990
Vol 250, Issue 4980
pp. 533-538

Abstract

The potassium channels encoded by the Drosophila Shaker gene activate and inactivate rapidly when the membrane potential becomes more positive. Site-directed mutagenesis and single-channel patch-clamp recording were used to explore the molecular transitions that underlie inactivation in Shaker potassium channels expressed in Xenopus oocytes. A region near the amino terminus with an important role in inactivation has now been identified. The results suggest a model where this region forms a cytoplasmic domain that interacts with the open channel to cause inactivation.

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Published In

Science
Volume 250 | Issue 4980
26 October 1990

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Published in print: 26 October 1990

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Authors

Affiliations

Toshinori Hoshi
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305.
William N. Zagotta
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305.
Richard W. Aldrich
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305.

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