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Identification of p53 Gene Mutations in Bladder Cancers and Urine Samples

Science
3 May 1991
Vol 252, Issue 5006
pp. 706-709

Abstract

Although bladder cancers are very common, little is known about their molecular pathogenesis. In this study, invasive bladder cancers were evaluated for the presence of gene mutations in the p53 suppressor gene. Of 18 tumors evaluated, 11 (61 percent) were found to have genetic alterations of p53. The alterations included ten point mutations resulting in single amino acid substitutions, and one 24-base pair deletion. In all but one case, the mutations were associated with chromosome 17p allelic deletions, leaving the cells with only mutant forms of the p53 gene product. Through the use of the polymerase chain reaction and oligomer-specific hybridization, p53 mutations were identified in 1 to 7 percent of the cells within the urine sediment of each of three patients tested. The p53 mutations are the first genetic alterations demonstrated to occur in a high proportion of primary invasive bladder cancers. Detection of such mutations ex vivo has clinical implications for monitoring individuals whose tumor cells are shed extracorporeally.

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Published In

Science
Volume 252 | Issue 5006
3 May 1991

Submission history

Published in print: 3 May 1991

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Authors

Affiliations

David Sidransky
Department of Oncology, Johns Hopkins University, Baltimore, MD 21231.
Andrew Von Eschenbach
Department of Urology, M. D. Anderson Hospital Houston, TX 77054.
Yvonne C. Tsai
Kenneth J. Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90033.
Peter Jones
Kenneth J. Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90033.
Ian Summerhayes
Department of Surgery, Harvard Medical School, Boston, MA 02115.
Fray Marshall
Department of Urology, Johns Hopkins University, Baltimore, MD 21215.
Meera Paul
Department of Oncology, Johns Hopkins University, Baltimore, MD 21231.
Pearl Green
Department of Pathology, Johns Hopkins University, Baltimore, MD 21231.
Stanley R. Hamilton
Department of Pathology, Johns Hopkins University, Baltimore, MD 21231.
Philip Frost
Department of Cell Biology, M. D. Anderson Hospital Houston, TX 77054.
Bert Vogelstein
Department of Oncology, Johns Hopkins University, Baltimore, MD 21231.

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