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Abstract

Interleukin-8 (IL-8) is an inflammatory cytokine that activates neutrophil chemotaxis, degranulation, and the respiratory burst. Neutrophils express receptors for IL-8 that are coupled to guanine nucleotide-binding proteins (G proteins); binding of IL-8 to its receptor induces the mobilization of intracellular calcium stores. A cDNA clone from HL-60 neutrophils, designated p2, has now been isolated that encodes a human IL-8 receptor. When p2 is expressed in oocytes from Xenopus laevis, the oocytes bind 125I-labeled IL-8 specifically and respond to IL-8 by mobilizing calcium stores with an EC50 of 20 nM. This IL-8 receptor has 77% amino acid identity with a second human neutrophil receptor isotype that binds IL-8 with higher affinity. It also exhibits 69% amino acid identity with a protein reported to be an N-formyl peptide receptor from rabbit neutrophils, but less than 30% identity with all other known G protein-coupled receptors, including the human N-formyl peptide receptor.

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References

BESEMER, J, SPECIFIC BINDING, INTERNALIZATION, AND DEGRADATION OF HUMAN NEUTROPHIL ACTIVATING FACTOR BY HUMAN POLYMORPHONUCLEAR LEUKOCYTES, JOURNAL OF BIOLOGICAL CHEMISTRY 264: 17409 (1989).
BONNER, T.I., CLONING AND EXPRESSION OF THE HUMAN AND RAT M5 MUSCARINIC ACETYLCHOLINE-RECEPTOR GENES, NEURON 1: 403 (1988).
BOULAY, F, SYNTHESIS AND USE OF A NOVEL N-FORMYL PEPTIDE DERIVATIVE TO ISOLATE A HUMAN N-FORMYL PEPTIDE RECEPTOR CDNA, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 168: 1103 (1990).
DEVEREUX, J, NUCLEIC ACIDS RESEARCH 12: 387 (1984).
GERARD, N.P., THE CHEMOTACTIC RECEPTOR FOR HUMAN-C5A ANAPHYLATOXIN, NATURE 349: 614 (1991).
GROB, P.M., CHARACTERIZATION OF A RECEPTOR FOR HUMAN MONOCYTE-DERIVED NEUTROPHIL CHEMOTACTIC FACTOR INTERLEUKIN-8, JOURNAL OF BIOLOGICAL CHEMISTRY 265: 8311 (1990).
HOLMES, W.E., STRUCTURE AND FUNCTIONAL EXPRESSION OF A HUMAN INTERLEUKIN-8 RECEPTOR, SCIENCE 253: 1278 (1991).
JULIUS, D, MOLECULAR CHARACTERIZATION OF A FUNCTIONAL CDNA-ENCODING THE SEROTONIN 1C RECEPTOR, SCIENCE 241: 558 (1988).
KOZAK, M, AN ANALYSIS OF 5'-NONCODING SEQUENCES FROM 699 VERTEBRATE MESSENGER-RNAS, NUCLEIC ACIDS RESEARCH 15: 8125 (1987).
KYTE, J, A SIMPLE METHOD FOR DISPLAYING THE HYDROPATHIC CHARACTER OF A PROTEIN, JOURNAL OF MOLECULAR BIOLOGY 157: 105 (1982).
LEONARD, E.J., LEUKOCYTE SPECIFICITY AND BINDING OF HUMAN NEUTROPHIL ATTRACTANT ACTIVATION PROTEIN-1, JOURNAL OF IMMUNOLOGY 144: 1323 (1990).
MALECH, H.L., ASPARAGINE-LINKED OLIGOSACCHARIDES ON FORMYL PEPTIDE CHEMOTACTIC RECEPTORS OF HUMAN PHAGOCYTIC-CELLS, JOURNAL OF BIOLOGICAL CHEMISTRY 260: 2509 (1985).
MOSER, B, NEUTROPHIL-ACTIVATING PEPTIDE-2 AND GRO MELANOMA GROWTH-STIMULATORY ACTIVITY INTERACT WITH NEUTROPHIL-ACTIVATING PEPTIDE-1/INTERLEUKIN-8 RECEPTORS ON HUMAN NEUTROPHILS, JOURNAL OF BIOLOGICAL CHEMISTRY 266: 10666 (1991).
MOSER, B, NEUTROPHIL-ACTIVATING PROPERTIES OF THE MELANOMA GROWTH STIMULATORY ACTIVITY, JOURNAL OF EXPERIMENTAL MEDICINE 171: 1797 (1990).
MURPHY, P.M., CHARACTERIZATION OF PHAGOCYTE-P2 NUCLEOTIDE RECEPTORS EXPRESSED IN XENOPUS OOCYTES, JOURNAL OF BIOLOGICAL CHEMISTRY 265: 11615 (1990).
MURPHY, P.M., FUNCTIONAL EXPRESSION OF THE HUMAN FORMYL PEPTIDE RECEPTOR IN XENOPUS OOCYTES REQUIRES A COMPLEMENTARY HUMAN FACTOR, JOURNAL OF BIOLOGICAL CHEMISTRY 266: 12560 (1991).
MURPHY, P.M., CHARACTERIZATION OF HUMAN PHAGOCYTIC CELL RECEPTORS FOR C5A AND PLATELET ACTIVATING FACTOR EXPRESSED IN XENOPUS OOCYTES, JOURNAL OF IMMUNOLOGY 145: 2227 (1990).
MURPHY, P.M., unpublished data.
OPPENHEIM, J.J., PROG CLIN BIOL RES 349: 405 (1990).
SAMANTA, A.K., IDENTIFICATION AND CHARACTERIZATION OF SPECIFIC RECEPTORS FOR MONOCYTE-DERIVED NEUTROPHIL CHEMOTACTIC FACTOR (MDNCF) ON HUMAN-NEUTROPHILS, JOURNAL OF EXPERIMENTAL MEDICINE 169: 1185 (1989).
SANGER, F, DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 74: 5463 (1977).
SULLIVAN, R, ACQUISITION OF FORMYL PEPTIDE RECEPTORS DURING NORMAL HUMAN MYELOID DIFFERENTIATION, BLOOD 70: 1222 (1987).
THELEN, M, MECHANISM OF NEUTROPHIL ACTIVATION BY NAF, A NOVEL MONOCYTE-DERIVED PEPTIDE AGONIST, FASEB JOURNAL 2: 2702 (1988).
THOMAS, K.M., MOLECULAR-CLONING OF THE FMET-LEU-PHE RECEPTOR FROM NEUTROPHILS, JOURNAL OF BIOLOGICAL CHEMISTRY 265: 20061 (1990).
WALZ, A, EFFECTS OF THE NEUTROPHIL-ACTIVATING PEPTIDE NAP-2, PLATELET BASIC-PROTEIN, CONNECTIVE TISSUE-ACTIVATING PEPTIDE-III, AND PLATELET FACTOR-4 ON HUMAN-NEUTROPHILS, JOURNAL OF EXPERIMENTAL MEDICINE 170: 1745 (1989).
WOLPE, S.D., MACROPHAGE INFLAMMATORY PROTEIN-1 AND PROTEIN-2 - MEMBERS OF A NOVEL SUPERFAMILY OF CYTOKINES, FASEB JOURNAL 3: 2565 (1989).
YOKOYAMA, S, ADAPTIVE EVOLUTION OF G-PROTEIN COUPLED RECEPTOR GENES, MOLECULAR BIOLOGY AND EVOLUTION 6: 342 (1989).

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Science
Volume 253 | Issue 5025
13 September 1991

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Published in print: 13 September 1991

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Philip M. Murphy
Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
H. Lee Tiffany
Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.

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