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A Gene Encoding an Antigen Recognized by Cytolytic T Lymphocytes on a Human Melanoma

Science
13 Dec 1991
Vol 254, Issue 5038
pp. 1643-1647

Abstract

Many human melanoma tumors express antigens that are recognized in vitro by cytolytic T lymphocytes (CTLs) derived from the tumor-bearing patient. A gene was identified that directed the expression of antigen MZ2-E on a human melanoma cell line. This gene shows no similarity to known sequences and belongs to a family of at least three genes. It is expressed by the original melanoma cells, other melanoma cell lines, and by some tumor cells of other histological types. No expression was observed in a panel of normal tissues. Antigen MZ2-E appears to be presented by HLA-A1; anti-MZ2-E CTLs of the original patient recognized two melanoma cell lines of other HLA-A1 patients that expressed the gene. Thus, precisely targeted immunotherapy directed against antigen MZ2-E could be provided to individuals identified by HLA typing and analysis of the RNA of a small tumor sample.

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Published In

Science
Volume 254 | Issue 5038
13 December 1991

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Published in print: 13 December 1991

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Authors

Affiliations

P. van der Bruggen
Ludwig Institute for Cancer Research, B-1200 Brussels, Belgium and Cellular Genetics Unit, Université Carholique de Louvain, Brussels, Belgium.
C. Traversari
Ludwig Institute for Cancer Research, B-1200 Brussels, Belgium and Cellular Genetics Unit, Université Carholique de Louvain, Brussels, Belgium.
P. Chomez
Ludwig Institute for Cancer Research, B-1200 Brussels, Belgium and Cellular Genetics Unit, Université Carholique de Louvain, Brussels, Belgium.
C. Lurquin
Ludwig Institute for Cancer Research, B-1200 Brussels, Belgium and Cellular Genetics Unit, Université Carholique de Louvain, Brussels, Belgium.
E. De Plaen
Ludwig Institute for Cancer Research, B-1200 Brussels, Belgium and Cellular Genetics Unit, Université Carholique de Louvain, Brussels, Belgium.
B. Van den Eynde
Ludwig Institute for Cancer Research, B-1200 Brussels, Belgium and Cellular Genetics Unit, Université Carholique de Louvain, Brussels, Belgium.
A. Knuth
I. Medizinische Klinik und Polilnik Johannes Gutenberg-Universität Mainz, D-6500 Mainz, Germany.
T. Boon
Ludwig Institute for Cancer Research, B-1200 Brussels, Belgium and Cellular Genetics Unit, Université Carholique de Louvain, Brussels, Belgium.

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