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Cell Sorting: Automated Separation of Mammalian Cells as a Function of Intracellular Fluorescence

Science
7 Nov 1969
Vol 166, Issue 3906
pp. 747-749

Abstract

A system for high-speed sorting of fluorescent cells was able to sort mouse spleen cells from Chinese hamster ovarian cells after development of fluorochromasia. Highly fluorescent fractions separated after similar treatment from mouse spleen cells immunized to sheep erythrocytes were enriched in antibody-producing cells by factors of 4 to 10.

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References

FULWYLER, M.J., ELECTRONIC SEPARATION OF BIOLOGICAL CELLS BY VOLUME, SCIENCE 150: 910 (1965).
Jerne, N. K., Cell Bound Anitibodies (1963).
KAMENTSKY, L.A., SPECTROPHOTOMETER - NEW INSTRUMENT FOR ULTRARAPID CELL ANALYSIS, SCIENCE 150: 630 (1965).
MISHELL, R.I., IMMUNIZATION OF DISSOCIATED SPLEEN CELL CULTURES FROM NORMAL MICE, JOURNAL OF EXPERIMENTAL MEDICINE 126: 423 (1967).
ROTMAN, B, MEMBRANE PROPERTIES OF LIVING MAMMALIAN CELLS AS STUDIED BY ENZYMATIC HYDROLYSIS OF FLUOROGENIC ESTERS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 55: 134 (1966).
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VANDILLA, M.A., CELL MICROFLUOROMETRY - A METHOD FOR RAPID FLUORESCENCE MEASUREMENT, SCIENCE 163: 1213 (1969).

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Published In

Science
Volume 166 | Issue 3906
7 November 1969

Submission history

Published in print: 7 November 1969

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Authors

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H. R. Hulett
Department of Getnetics, Statnford University School of Medicine, Stanford, California 94305
W. A. Bonner
Department of Getnetics, Statnford University School of Medicine, Stanford, California 94305
Janet Barrett
Department of Getnetics, Statnford University School of Medicine, Stanford, California 94305
Leonard A. Herzenberg
Department of Getnetics, Statnford University School of Medicine, Stanford, California 94305

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