Cell Sorting: Automated Separation of Mammalian Cells as a Function of Intracellular Fluorescence
Abstract
A system for high-speed sorting of fluorescent cells was able to sort mouse spleen cells from Chinese hamster ovarian cells after development of fluorochromasia. Highly fluorescent fractions separated after similar treatment from mouse spleen cells immunized to sheep erythrocytes were enriched in antibody-producing cells by factors of 4 to 10.
Get full access to this article
View all available purchase options and get full access to this article.
Already a subscriber or AAAS Member?Log In
References
FULWYLER, M.J., ELECTRONIC SEPARATION OF BIOLOGICAL CELLS BY VOLUME, SCIENCE 150: 910 (1965).
Jerne, N. K., Cell Bound Anitibodies (1963).
KAMENTSKY, L.A., SPECTROPHOTOMETER - NEW INSTRUMENT FOR ULTRARAPID CELL ANALYSIS, SCIENCE 150: 630 (1965).
MISHELL, R.I., IMMUNIZATION OF DISSOCIATED SPLEEN CELL CULTURES FROM NORMAL MICE, JOURNAL OF EXPERIMENTAL MEDICINE 126: 423 (1967).
ROTMAN, B, MEMBRANE PROPERTIES OF LIVING MAMMALIAN CELLS AS STUDIED BY ENZYMATIC HYDROLYSIS OF FLUOROGENIC ESTERS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 55: 134 (1966).
Sweet, R. O., Stanford University Technical Report 1722-1 (1964).
VANDILLA, M.A., CELL MICROFLUOROMETRY - A METHOD FOR RAPID FLUORESCENCE MEASUREMENT, SCIENCE 163: 1213 (1969).
Information & Authors
Information
Published In
Science
Volume 166 | Issue 3906
7 November 1969
7 November 1969
Copyright
© 1969.
Submission history
Published in print: 7 November 1969
Authors
Metrics & Citations
Metrics
Article Usage
Altmetrics
Citations
Export citation
Select the format you want to export the citation of this publication.
Cited by
- An interactive reference framework for modeling a dynamic immune system, Science, 349, 6244, (2021)./doi/10.1126/science.1259425
- Flow cytometry strikes gold, Science, 350, 6262, (739-740), (2015)./doi/10.1126/science.aad6770
- Quantitative Single Cell Analysis and Sorting, Science, 198, 4313, (149-157), (1977)./doi/10.1126/science.905822
Loading...
View Options
Get Access
Log in to view the full text
AAAS login provides access to Science for AAAS Members, and access to other journals in the Science family to users who have purchased individual subscriptions.
- Become a AAAS Member
- Activate your AAAS ID
- Purchase Access to Other Journals in the Science Family
- Account Help
Log in via OpenAthens.
Log in via Shibboleth.
More options
Purchase digital access to this article
Download and print this article for your personal scholarly, research, and educational use.
Buy a single issue of Science for just $15 USD.
View options
PDF format
Download this article as a PDF file
Download PDF