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Abstract

A short-arm rotor increases separation of viable mammalian cells, from mixtures, by low-speed centrifugation; continuous Ficoll density gradients in tissue-culture media are used. We describe the theory and experimental demonstration of the superior separation achieved with this new rotor.

References

NATIONAL CANCER INSTITUTE MONOGRAPHS 21 1 (1966).
ANDERSON, N.G., STUDIES ON ISOLATED CELL COMPONENTS .8. HIGH RESOLUTION GRADIENT DIFFERENTIAL CENTRIFUGATION, EXPERIMENTAL CELL RESEARCH 9: 446 (1955).
ANDERSON, N.G., ZONAL CENTRIFUGES AND OTHER SEPARATION SYSTEMS, SCIENCE 154: 103 (1966).
BOONE, C.W., RESOLUTION OF MIXTURES OF VIABLE MAMMALIAN CELLS INTO HOMOGENEOUS FRACTIONS BY ZONAL CENTRIFUGATION, JOURNAL OF CELL BIOLOGY 36: 369 (1968).
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Published In

Science
Volume 161 | Issue 3844
30 August 1968

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Published in print: 30 August 1968

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Thomas G. Pretlow, II
Viral Carcinogenesis Branch, National Cancer Institute, Bethesda, Maryland
Charles W. Boone
Viral Carcinogenesis Branch, National Cancer Institute, Bethesda, Maryland

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