Communication with Queen Honey Bees by Substrate Sound

A caged queen honey bee, installed in an observation hive which already contained a virgin queen, piped in response to artificial piping which was played to it through the substrate. The experiments which followed this observation provide the first direct quantitative evidence that sound, at least in the range of 600 to 2000 cycles per second, is perceived by honey bees and that information is transmitted through sound from one bee to another.