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Abstract

Id is a helix-loop-helix (HLH) protein that represses activity of several basic helix-loop-helix (bHLH) proteins involved in cell type—specific transcription and cell lineage commitment. The myeloid precursor cell line 32DCl3(G) expressed Id messenger RNA, which was transiently decreased when cells were induced to terminally differentiate with granulocyte—colony-stimulating factor. Concomitant with the decrease of Id messenger RNA was the appearance in nuclear extracts of DNA binding proteins that recognized a canonical E-box motif, a DNA binding site for some bHLH proteins. Constitutive expression of an Id complementary DNA in 32DCl3(G) cells blocked their ability to differentiate and to induce E-box-binding activity. These results suggest that Id and, hence, bHLH proteins function in the process of myeloid differentiation.

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Science
Volume 255 | Issue 5052
27 March 1992

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Published in print: 27 March 1992

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Brent L. Kreider
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Robert Benezra
Department of Genetics, Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Giovanni Rovera
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Tom Kadesch
Howard Hughes Medical Institute and the Department of Human Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104.

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