Higher-Order Cellular Information Processing with Synthetic RNA Devices
Abstract
The engineering of biological systems is anticipated to provide effective solutions to challenges that include energy and food production, environmental quality, and health and medicine. Our ability to transmit information to and from living systems, and to process and act on information inside cells, is critical to advancing the scale and complexity at which we can engineer, manipulate, and probe biological systems. We developed a general approach for assembling RNA devices that can execute higher-order cellular information processing operations from standard components. The engineered devices can function as logic gates (AND, NOR, NAND, or OR gates) and signal filters, and exhibit cooperativity. RNA devices process and transmit molecular inputs to targeted protein outputs, linking computation to gene expression and thus the potential to control cellular function.
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We thank Y. Chen, J. Liang, and D. Endy for critical reading of the manuscript and A. Babiskin for pRzS. This work was supported by the Center for Biological Circuit Design at the California Institute of Technology, the Arnold and Mabel Beckman Foundation, and the NIH. The authors declare competing financial interests in the form of a pending patent application.
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Published In
Science
Volume 322 | Issue 5900
17 October 2008
17 October 2008
Copyright
American Association for the Advancement of Science.
Submission history
Received: 12 May 2008
Accepted: 12 August 2008
Published in print: 17 October 2008
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