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Revisiting the Role of the Mother Centriole in Centriole Biogenesis

A. Rodrigues-Martins, M. Riparbelli, G. Callaini, D. M. Glover, and M. Bettencourt-Dias
Science • 18 May 2007 • Vol 316, Issue 5827 • pp. 1046-1050 • DOI: 10.1126/science.1142950
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Abstract

Centrioles duplicate once in each cell division cycle through so-called templated or canonical duplication. SAK, also called PLK4 (SAK/PLK4), a kinase implicated in tumor development, is an upstream regulator of canonical biogenesis necessary for centriole formation. We found that overexpression of SAK/PLK4 could induce amplification of centrioles in Drosophila embryos and their de novo formation in unfertilized eggs. Both processes required the activity of DSAS-6 and DSAS-4, two molecules required for canonical duplication. Thus, centriole biogenesis is a template-free self-assembly process triggered and regulated by molecules that ordinarily associate with the existing centriole. The mother centriole is not a bona fide template but a platform for a set of regulatory molecules that catalyzes and regulates daughter centriole assembly.
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We thank C. Ferreira and I. Ferreira for help with experiments; D. Johnston, R. Basto, and J. Raff for reagents; R. Martinho, J. Leal, M. Gatt, R. Kuriyama, and anonymous reviewers for comments on the manuscript; the Drosophila Instituto Gulbenkian de Ciência (IGC) community, G. Goshima, and the M.B.-D. and D.M.G. groups for discussions; thebestgene.com for making transgenic flies; and the IGC imaging unit for help with image acquisition. We are grateful for grants from Cancer Research UK, Fundação Calouste Gulbenkian, and Fundação para a Ciência e Tecnologia (POCI2010) and for an International Joint Project grant from the Royal Society for collaboration between the M.B.-D. and D.M.G. groups.

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Science
Volume 316 | Issue 5827
18 May 2007

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American Association for the Advancement of Science.

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Received: 23 March 2007
Accepted: 17 April 2007
Published in print: 18 May 2007

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A. Rodrigues-Martins
Instituto Gulbenkian de Ciência, Cell Cycle Regulation Laboratory, Rua da Quinta Grande, 6, P-2780-156 Oeiras, Portugal.
Cancer Research UK, Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
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M. Riparbelli
Department of Evolutionary Biology, University of Siena, Via A. Moro 4, I-53100 Siena, Italy.
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G. Callaini
Department of Evolutionary Biology, University of Siena, Via A. Moro 4, I-53100 Siena, Italy.
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D. M. Glover*
Cancer Research UK, Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
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M. Bettencourt-Dias*
Instituto Gulbenkian de Ciência, Cell Cycle Regulation Laboratory, Rua da Quinta Grande, 6, P-2780-156 Oeiras, Portugal.
Cancer Research UK, Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
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Notes

* To whom correspondence should be addressed. E-mail: [email protected] (M.B.-D.); [email protected] (D.M.G.)

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