Requirement of Cks2 for the First Metaphase/Anaphase Transition of Mammalian Meiosis
Abstract
We generated mice lacking Cks2, one of two mammalian homologs of the yeast Cdk1-binding proteins, Suc1 and Cks1, and found them to be viable but sterile in both sexes. Sterility is due to failure of both male and female germ cells to progress past the first meiotic metaphase. The chromosomal events up through the end of prophase I are normal in both CKS2–/– males and females, suggesting that the phenotype is due directly to failure to enter anaphase and not a consequence of a checkpoint-mediated metaphase I arrest.
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C.H.S. is supportedby a special fellow awardfrom the Leukemia and Lymphoma Society of America. We are grateful to C. Palancar for help with mouse husbandry andgenotyping, M. A. Handel for the kindgift of the Cor1-specific antibody, and A. D. Pyle, P. B. Moens, and P. A. Hunt for useful protocols andadvice. This work was supportedby NIH grants HD22681 (R.M.S.), HD38252 (P.J.D.), and CA74224 (S.I.R.).
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Science
Volume 300 | Issue 5619
25 April 2003
25 April 2003
Copyright
American Association for the Advancement of Science.
Submission history
Received: 4 March 2003
Accepted: 24 March 2003
Published in print: 25 April 2003
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