Modulation of Cell Proliferation by Heterotrimeric G Protein in Arabidopsis
Abstract
The α subunit of a prototypical heterotrimeric GTP-binding protein (G protein), which is encoded by a single gene (GPA1) in Arabidopsis, is a modulator of plant cell proliferation. gpa1 null mutants have reduced cell division in aerial tissues throughout development. Inducible overexpression of GPA1 in Arabidopsis confers inducible ectopic cell division. GPA1 overexpression in synchronized BY-2 cells causes premature advance of the nuclear cycle and the premature appearance of a division wall. Results from loss of function and ectopic expression and activation of GPA1indicate that this subunit is a positive modulator of cell division in plants.
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REFERENCES AND NOTES
1
Gutkind J. S., J. Biol. Chem. 273, 1839 (1998).
2
Dhanasekaran N., Tsim S.-T., Dermott J. M., Onesime D., Oncogene 17, 1383 (1998).
3
Selbie L. A., Hill S. J., Trends Pharmacol. Sci. 19, 89 (1998).
4
Dhanasekaran N., Heasley L. E., Johnson G. L., Endocr. Rev. 16, 259 (1995).
5
Watkins D. C., Johnson G. L., Malbon C. C., Science 258, 1373 (1992).
6
LaMorte V. J., Goldsmith P. K., Spiegel A. M., Meinkoth J. L., Feramisco J. R., J. Biol. Chem. 267, 691 (1992).
7
D. Francis, D. Dudits, D. Inze, Eds., Plant Cell Division (Portland Press Research Monograph, London, 1998).
8
Ma H., Plant Mol. Biol. 26, 1611 (1994).
9
Ashikari M., Wu J., Yano M., Sasaki T., Yoshimura A., Proc. Natl. Acad. Sci. U.S.A. 96, 10284 (1999).
10
T-DNA–tagged Arabidopsis mutants (60,480) (31) were generated at the University of Wisconsin Knockout Arabidopsis facility (www.biotech.wisc.edu/Arabidopsis/) and screened for insertions. Primers specific for the T-DNA left-border (5′-CATTTTATAATAACGCTGCGGACATCTAC-3′) and the T-DNA right-border (5′-TGGGAAAACCTGGCGTTACCCAACTTAAT-3′) were used in tandem with Gα-specific primers (forward: 5′-GGACCTTTCGGCGTAATTTCGTCTTCCC-3′; and reverse: 5′-CTAGTCGTAGCCATCGAGACACATTAGA-3′) for identification and isolation of the mutant lines.
11
Sprang S. R., Annu. Rev. Biochem. 66, 639 (1997).
12
A. H. Haber, Am. J. Bot.49,583 (1962).
13
Hemerly A., et al., EMBO J. 14, 3925 (1995).
14
Jones A. M., et al., Science 282, 1114 (1998).
15
Gendreau E., et al., Plant Physiol. 114, 295 (1997).
16
Tsuge T., Tsukaya H., Uchimiya H., Development 122, 1589 (1996).
17
Kim G.-T., Tsukaya H., Saito Y., Uchimiya H., Proc. Natl. Acad. Sci. U.S.A. 96, 9433 (1999).
18
WS, gpa1-1, and gpa1-2 seedlings were grown for 2 days in the dark on various concentrations of brassinolide (BR, 0 to 10 μM). WS and gpa1 hypocotyl growth was inhibited by BR to 45% and 73% of the control lengths, respectively.
19
cyc1At-CDBGUS contains Arabidopsis cyc1At promoter and 5′ portion of the cyclin coding region fused in-frame to the reporter β-glucuronidase (GUS) gene. The fusion contains sequences encoding the cyclin destruction box (CDB). cyc1At-CDBGUS plants were crossed into gpa1 mutant plants. gpa1 mutants were selected from a F2 population grown for 2 days in dark. The selected plants were allowed to grow for an additional 48 to 72 hours in light before staining. The seedlings were stained for GUS activity.
20
Donnelly P. M., Bonetta D., Tsukaya H., Dengler R. E., Dengler N. G., Dev. Biol. 215, 407 (1999).
21
Vernoux T., et al., Plant Cell 12, 97 (2000).
22
Supplementary data are available on Science Online at www.sciencemag.org/cgi/content/full/292/5524/2066/DC1.
23
G. Liebmann, F. D. Böhmer Curr. Med. Chem.7, 911 (2000).
24
Nishida M., et al., Nature 408, 492 (2000).
25
Lopez I., Mak E. C., Ding J., Hamm H. E., Lomasney J. W., J. Biol. Chem. 276, 2758 (2001).
26
Lambright D. G., et al., Nature 379, 311 (1996).
27
Sondek J., Bohm A., Lambright D. G., Hamm H. E., Sigler P. B., Nature 379, 369 (1996).
28
Kovtun Y., Chiu W.-L., Zeng W., Sheen J., Nature 395, 716 (1998).
29
Jelacic T. M., Kennedy M. E., Wickman K., Clapham D. E., J. Biol. Chem. 275, 36211 (2000).
30
Wang X.-Q., Ullah H., Jones A. M., Assmann S. M., Science 292, 2070 (2001).
31
Krysan P. J., Young J. C., Sussman M. R., Plant Cell 11, 2283 (1999).
32
Weiss C. A., White E., Huang H., Ma H., FEBS Lett. 407, 361 (1997).
33
We thank J. Celenza (Boston University) for the mitotic reporter (cyc1At-CDB-GUS) plants and H. Ma (Penn State University) for antiserum to GPA1 and GPA1 cDNA. We gratefully acknowledge the support of the National Science Foundation, Integrative Plant Sciences. K.-H.I. was supported by a grant to A.M.J. from the U.S. Department of Agriculture.
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Science
Volume 292 | Issue 5524
15 June 2001
15 June 2001
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Received: 16 January 2001
Accepted: 6 April 2001
Published in print: 15 June 2001
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