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Harnessing CRISPR-Cas9 technology for cancer therapeutics has been hampered by low
editing efficiency in tumors and potential toxicity of existing delivery systems.
Here, we describe a safe and efficient lipid nanoparticle (LNP) for the delivery of
Cas9 ...
Immune-orthogonal Cas9 allows for genome editing in pre-immunized mice.
Site-specific chemical conjugation of proteins can enhance their therapeutic and diagnostic
utility but has seldom been applied to CRISPR-Cas9, which is a rapidly growing field
with great therapeutic potential. The low efficiency of homology-directed ...
We report a dissolvable microneedle (MN) patch that can mediate transdermal codelivery
of CRISPR-Cas9–based genome-editing agents and glucocorticoids for the effective treatment
of inflammatory skin disorders (ISDs). The MN is loaded with polymer-...
We report a robust, versatile approach called CRISPR live-cell fluorescent in situ
hybridization (LiveFISH) using fluorescent oligonucleotides for genome tracking in
a broad range of cell types, including primary cells. An intrinsic stability switch
of ...
Although Cas9-mediated genome editing has been widely used to engineer alleles in
animal models of human inherited diseases, very few homology-directed repair (HDR)–based
genetic editing systems have been established in postnatal mouse models for ...
Amyotrophic lateral sclerosis (ALS) is a fatal and incurable neurodegenerative disease
characterized by the progressive loss of motor neurons in the spinal cord and brain.
In particular, autosomal dominant mutations in the superoxide dismutase 1 (SOD1) ...
Multicellular systems develop from single cells through distinct lineages. However,
current lineage-tracing approaches scale poorly to whole, complex organisms. Here,
we use genome editing to progressively introduce and accumulate diverse mutations
in a ...
Sparking thoughts of CRISPR’s beginnings, the genetic elements called retrons can only edit single-cell organisms so far
In utero CRISPR editing allows early intervention for inborn errors of metabolism
in mice.
CRISPR-Cas9–mediated homology-directed DNA repair is the method of choice for precise
gene editing in a wide range of model organisms, including mouse and human. Broad
use by the biomedical community refined the method, making it more efficient and ...
Genome editing with CRISPR/Cas9 is a promising new approach for correcting or mitigating
disease-causing mutations. Duchenne muscular dystrophy (DMD) is associated with lethal
degeneration of cardiac and skeletal muscle caused by more than 3000 different ...
Business Office Feature
Technology Feature | Beyond CRISPR: What’s current and upcoming in genome editing
Gene editing gets an upgrade with new versions of CRISPR and CRISPR alternatives (zinc-finger nucleases, TALENs, and meganucleases).